Rat cyclooxygenase-1 (COX-1) ELISA KitRat
The kit is for the quantitative level of COX-1 in the sample, adopt purified Rat COX-1 to coat microtiter plate, make solid-phase antibody, then add COX-1 to wells, Combine COX-1 antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of COX-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Detection range of the kit is: 15pg/ml -900 pg/ml
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