Search，Order，Antibody,ELISA Kit & Protein

l   Operation Instruction of Kit for Quantitative Determination by Enzyme Linked Immunosorbent Assay

l   Detection of species: Rat

l   Detection medium:Serum&Plasma&Tissue homogenate&Cell culture medium&Tissue

Principle of test

　　The kit assay Rat IP-10 level in the sample，use Purified Rat IP-10 antibody to coat microtiter plate wells, make solid-phase antibody, then add IP-10 to wells, Combined IP-10 antibody which With HRP labeled ,become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IP-10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.