Rat Adiponectin(ADPN) ELISA KitRat
For the quantitative determination of Rat ADP concentrations.
Detection of species: Rat
Detection medium: Serum&Plasma&Tissue homogenate&Cell culture medium&Tissue
The kit is for the quantitative level of ADP in the sample, adopt purified Rat ADP to coat microtiter plate, make solid-phase
antibody, then add ADP to wells, Combine ADP antibody with labeled HRP to form antibody-antigen -enzyme-antibody
complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP
enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of ADP in the samples is then determined by comparing the O.D. of the samples
to the standard curve.
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