Rat Placenta Growth Factor (PLGF) ELISA KitRat
l For the quantitative determination of Rat PLGF concentrations.
l Detection of species: Rat
l Detection medium: serum, plasma, tissue homogenates, cell culture supernates,urine
The kit is for the quantitative level of PLGF in the sample, adopt purified Rat PLGF to coat microtiter plate, make solid-phase antibody, then add PLGF to wells, Combine PLGF antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of PLGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Detection range of the kit is: 1.6ng/L -70ng/L
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