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Rat colon cancer-specific antigen-4 (CCSA-4) ELISA Kit

Rat
Rat colon cancer-specific antigen-4 (CCSA-4) ELISA Kit
Rat colon cancer-specific antigen-4 (CCSA-4) ELISA Kit

The kit assay Rat CCSA-4 level in the sample,use Purified 

Rat CCSA-4 antibody to coat microtiter plate wells, 

make solid-phase antibody, then add CCSA-4 to wells, 

Combined CCSA-4 antibody which With HRP labeled , 

become antibody - antigen - enzyme-antibody complex, 

after washing Completely, Add TMB substrate solution,

TMB substrate becomes blue color At HRP enzyme-catalyzed, 

reaction is terminated by the addition of a sulphuric acid solution 

and the color change is measured spectrophotometrically at a 

wavelength of 450 nm. The concentration of CCSA-4 in the samples 

is then determined by comparing the O.D. of the samples to the 

standard curve.

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