Rat Hypoxia-inducible Factor 1 (HIF-1) ELISA KitRat
l Operation Instruction of Kit for Quantitative Determination by Enzyme Linked Immunosorbent Assay
l Detection of species: Rat
l Detection medium:Serum&Plasma&Tissue homogenate&Cell culture medium&Tissue
Principle of test
The kit assayRat HIF-1 level in the sample，use Purified Rat HIF-1 antibody to coat microtiter plate wells, make solid-phase antibody, then add HIF-1 to wells, Combined HIF-1 antibody which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HIF-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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