Search，Order，Antibody,ELISA Kit & Protein

l   Operation Instruction of Kit for Quantitative Determination by Enzyme Linked Immunosorbent Assay

l   Detection of species: Rat

l   Detection medium:Serum&Plasma&Tissue homogenate&Cell culture medium&Tissue

Principle of test

　　The kit assayRat HIF-1 level in the sample，use Purified Rat HIF-1 antibody to coat microtiter plate wells, make solid-phase antibody, then add HIF-1 to wells, Combined  HIF-1 antibody which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HIF-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.