Mouse eosinophil chemotactic factor (ECF) ELISA KitMouse
l For the quantitative determination of Mouse ECF concentrations.
l Detection of species: Mouse
l Detection medium: serum, plasma, tissue sample, cell culture supernates.
Principle of test
The kit is for the quantitative level of ECF in the sample, adopt purified Mouse ECF to coat microtiter plate, make solid-phase antibody, then add samples or standards to wells with a labeled antibody specific to ECF, then add labeled HRP to the well. After washing completely, add TMB substrate solution, TMB substrate becomes blue color in wells that contains antibody - antigen - enzyme-antibody complex, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of ECF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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