Mouse anti-Thyroid-Peroxidase antibody (TPO-Ab) ELISA Kit,TPO-Ab ELISA KitMouse
serum, plasma, tissue homogenates, cell culture supernates
Principle of test
The kit is for the quantitative level of TPO-AB in the sample, adopt purified Human TPO-AB to coat microtiter plate, make solid-phase antibody, then add TPO-AB to wells, Combine TPO-AB antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of TPO-AB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Chromogen Solution A(1×6ml)
Chromogen Solution B(1×6ml)
Wash Solution(1×20ml×30 fold)
For Research Use Only!
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