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l   For the quantitative determination of Human YKL-40/CHI3L1 concentrations.

l   Detection of species: Human

l   Detection medium: serum, plasma, tissue homogenates, cell culture supernates

Principle of test

The kit assay Human YKL-40/CHI3L1 level in the sample，use Purified Human YKL-40/CHI3L1 to coat microtiter plate wells, make solid-phase antibody, then add YKL-40/CHI3L1 to wells, Combined YKL-40/CHI3L1 which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of YKL-40/CHI3L1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Composition of the kit