Human Prostaglandin E2 receptor 1 (PGER1) ELISA KitHuman
serum, plasma, tissue homogenates, cell culture supernates
Principle of test
The kit is for the quantitative level of PGER1 in the sample, adopt purified Human PGER1 to coat microtiter plate, make solid-phase antibody, then add PGER1 to wells, Combine PGER1 antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of PGER1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Chromogen Solution A(1×6ml)
Chromogen Solution B(1×6ml)
Wash Solution(1×20ml×30 fold)
For Research Use Only!
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