Human Pre B cell enhancing factor (PBEF) ELISA Kit
HumanSamples | serum, plasma, tissue homogenates, cell culture supernates |
Principle of test | The kit is for the quantitative level of PBEF in the sample, adopt purified Human PBEF to coat microtiter plate, make solid-phase antibody, then add PBEF to wells, Combine PBEF antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of PBEF in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
Kit Components | Microelisa Stripplate(12well×8strips) Standard (1×0.5ml) Standard Diluent(1×1.5ml) HRP-Conjugate Reagent(1×6ml) Sample Diluent(1×6ml) Chromogen Solution A(1×6ml) Chromogen Solution B(1×6ml) Stop Solution(1×6ml) Wash Solution(1×20ml×30 fold) User manual(1) Adhesive Strip(2) |
USAGE | For Research Use Only! |
Product No.:11561
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