Search，Order，Antibody,ELISA Kit & Protein

The kit assay Human CA153 level in the sample，use Purified Human CA153 antibody to coat microtiter plate wells, make solid-phase antibody, then add CA153 to wells, Combined CA153 antibody which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CA153 in the samples is then determined by comparing the O.D. of the samples to the standard curve 

DETECTION RANGE
Detection range of the kit is: 1.2pg/ml-45pg/ml