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Human 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase(ADI1) ELISA Kit

Human
Human 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase(ADI1) ELISA Kit
Human 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase(ADI1) ELISA Kit

The kit is for the quantitative level of ADI1 in the sample, adopt purified human ADI1 to coat microtiter plate, 

make solid-phase antibody, then add samples or standards to wells with a labeled antibody specific to ADI1, 

then add labeled HRP to the well. After washing completely, add TMB substrate solution, TMB substrate 

becomes blue color in wells that contains antibody - antigen - enzyme-antibody complex, reaction is

terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. 

The concentration of ADI1 in the samples is then determined by comparing the O.D. of the samples to the 

standard curve. 

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