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Human Thiamine ELISA Kit

Human
Human Thiamine ELISA Kit
Human Thiamine ELISA Kit

For the quantitative level of Human Thiamine level in the sample, adopt Purified Human Thiamine antibody to coat microtiter plate wells

make solid-phase antibody, then Pipette Thiamine to wells, Combined Thiamine antibody which With labeled HRP to form antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, 

reaction is terminated by the addition of a solution and the color change is measured at a wavelength of 450 nm. 

The concentration of PAG in the samples is then determined by comparing the O.D. of the samples to the standard curve. 

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