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For the quantitative determination of Human NPY concentrations.

Detection of species: Human

Detection medium: serum, plasma, tissue sample, cell culture supernates

The kit assay Human NPY level in the sample，use Purified Human NPY antibody to coat microtiter plate wells, make

solid-phase antibody, then add NPY to wells, Combined NPY antibody which With HRP labeled， become antibody -

antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue

color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is

measured spectrophotometrically at a wavelength of 450 nm. The concentration of NPY in the samples is then determined

by comparing the O.D. of the samples to the standard curve