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Human Nitric Oxide(NO) ELISA Kit

Human
Human Nitric Oxide(NO) ELISA Kit
Human Nitric Oxide(NO) ELISA Kit

l   For the quantitative determination of human NOconcentrations.

l   Detection of species: Human

l   Detection medium: serum, plasma, tissue homogenates, cell culture supernates,urine

 

Principle of test

The kit is for the quantitative level of NO in the sample, adopt purified human NO to coat microtiter plate, make solid-phase antibody, then add NO to wells, Combine NO antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of NO in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

Composition of the kit

Reagent

Quantity

Microelisa Stripplate

12well×8strips

Standard: 225μmol/L

1×0.5ml

Standard Diluent

1×1.5ml

HRP-Conjugate Reagent

1×6ml

Sample Diluent

1×6ml

Chromogen Solution A

1×6ml

Chromogen Solution B

1×6ml

Stop Solution

1×6ml

Wash Solution

1×20ml×30 fold

User manual

1

Adhesive Strip

2

 


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Product No.:A1034

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