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This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate

provided in this kit has been pre-coated with an antigen specific to CHIKV-IgG.

Standards or samples are added to the appropriate Microelisa stripplate wells and

combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated

antigen specific for CHIKV-IgG is added to each Microelisa stripplate well and incubated.

Free components are washed away. The TMB substrate solution is added to each well.

Only those wells that contain CHIKV-IgG and HRP conjugated CHIKV-IgG antigen will

appear blue in color and then turn yellow after the addition of the stop solution. The

optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm.

The presence of CHIKV-IgG is determined by comparing with the CUTOFF value.