Search，Order，Antibody,ELISA Kit & Protein

The kit assay Human ALA/LCA level in the sample，use Purified Human ALA/LCA antigen to coat microtiter plate wells,

make solid-phase antigen, then add ALA/LCA Ab to wells, Combined With ALA/LCA , after washing and removing

non-combinative antibody and other components ,then Combined ALA/LCA antigen which with HRP labeled become

antigen – antibody - enzyme-antigen complex, after washing Completely, Add TMB substrate solution,, TMB substrate

becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the

color change is measured spectrophotometrically at a wavelength of 450 nm. Compared with the CUTOFF value, according

to this to judge ALA/LCA exist in the sample or not.