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The kit is for the quantitative level of Pentosidine in the sample, adopt purified human

Pentosidine to coat microtiter plate, make solid-phase antibody, then add samples or

standards to wells with a labeled antibody specific to Pentosidine, then add labeled

HRP to the well. After washing completely, add TMB substrate solution, TMB

substrate becomes blue color in wells that contains antibody - antigen -

enzyme-antibody complex, reaction is terminated by the addition of a stop solution

and the color change is measured at a wavelength of 450 nm. The concentration of

Pentosidine in the samples is then determined by comparing the O.D. of the samples to

the standard curve.