Human Interleukin 5 (IL-5) ELISA KitHuman
The kit assay Human IL-5 level in the sample，use Purified Human IL-5antibody to coat microtiter plate wells, make solid-phase antibody, then add IL-5 to wells, Combined antibody which With HRP labeled goat anti-Human become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IL-5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Detection range of the kit is: 1ng/L -20 ng/L
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