Search，Order，Antibody,ELISA Kit & Protein

The kit is for the quantitative level of IL-18 in the sample, adopt purified human IL-18 to coat microtiter plate, make solid-phase antibody, then add IL-18 to wells, Combine IL-18 antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of IL-18 in the samples is then determined by comparing the O.D. of the samples to the standard curve. 

DETECTION RANGE

Detection range of the kit is: 5ng/L -100ng/L