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l  For the quantitative determination of human irisin concentrations.

l  Detection of species: Human

l  Detection medium: serum, cell culture supernates and other biological fluids

Principle of test

The kit is for the quantitative level of irisin in the sample, adopt purified human irisin to coat microtiter plate, make solid-phase antibody, then add samples or standards to wells with a labeled antibody specific to irisin, then add labeled HRP to the well. After washing completely, add TMB substrate solution, TMB substrate becomes blue color in wells that contains antibody - antigen - enzyme-antibody complex, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of irisin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Composition of the kit