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Human CXC-chemokine ligand 13(CXCL-13) ELISA Kit

Human
Human CXC-chemokine ligand 13(CXCL-13) ELISA Kit
Human CXC-chemokine ligand 13(CXCL-13) ELISA Kit

l   For the quantitative determination of human CXCL-13 concentrations.

l   Detection of species: Human

l   Detection medium: serum, plasma, tissue homogenates, cell culture supernates

 

Principle of test

The kit is for the quantitative level of CXCL-13 in the sample, adopt purified human CXCL-13 to coat microtiter plate, make solid-phase antibody, then add CXCL-13 to wells, Combine SOST antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of CXCL-13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

Composition of the kit

Reagent

Quantity

Microelisa Stripplate(quick removable plastic sheet)

12well×8strips

Standard: 270pg/ml

1×0.5ml

Standard Diluent

1×1.5ml

HRP-Conjugate Reagent

1×6ml

Sample Diluent

1×6ml

Chromogen Solution A

1×6ml

Chromogen Solution B

1×6ml

Stop Solution

1×6ml

Wash Solution

1×20ml×30 fold

User manual

1

Adhesive Strip

2

                    


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Product No.:95203

USD
11至15个工作日送达

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