Human Basic Fibroblast Growth Factor (bFGF) ELISA KitHuman
The kit is for the quantitative level of bFGF in the sample, adopt purified Human bFGF to coat microtiter plate, make solid-phase antibody, then add bFGF to wells, Combine bFGF antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of bFGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Detection range of the kit is: 5pg/ml-1000pg/ml
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