Are you finding the correct kit to measure in vitro quantitative measurement of GPC3 in plasma, biological fluid and serum? With Rat Glypican-3 (GPC-3) ELISA Kit you can get the correct quantity measurements.

The assay that is used has high specificity and sensitivity in regards to detection of Glypican 3 (GPC3). Also, during experimentation, no interference was observed between analogues and Glypican 3 (GPC3). With a sensitivity of 0.055 ng/mL, this has a detection range of 0.15-10 ng/mL.

Key contents of this product:

·         Standard (lyophilized)

·         TMB Substrate

·         Wash Buffer

·         Assay Diluent A

·         Detection Reagent A- green

·         Detection Reagent B- red

·         Plate sealer for 96 wells

·         Pre-coated, ready to use 96-well strip plate

After this whole process, a reagent process has to be done to derive final results with help of this Rat Glypican-3 (GPC-3) ELISA Kit.

Preparation of reagent:

·         Standard form: This is to be reconstituted with 1.0mL of Standard Diluent, and after keeping it in room temperature for 10 minutes, it is shaken slowly. Such 7 tubes containing 0.5mL Standard Diluent is to be prepared. Each of these tubes are to be mixed with each other.

·         Assay Diluent A and Assay Diluent B: Both these diluents are to be mixed for further results.

·         Detection Reagent A and B: With a ratio of 1:100, centrifuging is to be done.

·         Wash Solution: This solution is to be diluted.

·         TMB Substrate: Required dosage of solution is to be done with help of sterilised tips.

So, one can get maximum benefit by using this Rat Glypican-3 (GPC-3) ELISA Kit in case of research work.