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Human  Vascuoar endothelial cell growth factor receptor ELISA kit

The kit is for the quantitative level of VEGF in the sample, adopt purified human VEGF to coat microtiter plate, make solid-phase antibody, then add samples or standards to wells with a labeled antibody specific to VEGF, then add labeled HRP to the well. After washing completely, add TMB substrate solution, TMB substrate becomes blue color in wells that contains antibody - antigen - enzyme-antibody complex, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of VEGF in the samples is then determined by comparing the O.D. of the samples to the standard curve