Search，Order，Antibody,ELISA Kit & Protein

PRINCIPLE OF TEST
The kit is for the quantitative level of FIX in the sample, adopt purified Mouse FIX to coat microtiter plate, make solid-phase antibody, then add FIX to wells, Combine FIX antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of FIX in the samples is then determined by comparing the O.D. of the samples to the standard curve.
DETECTION RANGE
Detection range of the kit is: 6μg/L -220μg/L