Search，Order，Antibody,ELISA Kit & Protein

DETECTION RANGE
Detection range of the kit is: 1.5 ng/ml -50 ng/ml
PRINCIPLE OF TEST
The kit is for the quantitative level of ALP in the sample, adopt purified Mouse ALP to coat microtiter plate, make solid-phase antibody, then add samples or standards to wells with a labeled antibody specific to ALP, then add labeled HRP to the well. After washing completely, add TMB substrate solution, TMB substrate becomes blue color in wells that contains antibody - antigen - enzyme-antibody complex, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of ALP in the samples is then determined by comparing the O.D. of the samples to the standard curve.