Bovine reduced Glutathione (GSH) ELISA KitBovine
The kit is for the quantitative level of GSH in the sample, adopt purified Bovine GSH to coat microtiter
plate, make solid-phase antibody, then add GSH to wells, Combine GSH antibody with labeled HRP
to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate
solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the
addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration
of GSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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