By using the Human Suppressors of Cytokine Signaling 3(SOCS3) ELISA Kit a person can check out the cytokine content in the human body. With 96 strip wells, and detection range of 15.625-1000pg⁄mL, it can be used for checking out the content in biological fluids, cell lysates and tissue homogenates.

Sample type:

The samples used in this case are taken and stored for a period of 30 minutes before being centrifuged for a period of 15 minutes. This has specific sensitivity for Suppressors Of Cytokine Signaling 3 (SOCS3). With a sensitivity of 11.75 pg/ml, this helps in noting the growth factor and growth regulation.

Summary of assay procedure:

In case of Human Suppressors of Cytokine Signaling 3(SOCS3) ELISA Kit a certain technique is followed.

·         In the prepared reagents, 100µL standard is added.

·         After this, it is incubated for a period of 2 hours at 37°C.

·         This solution is to be aspirated and added 100µL of Detection Reagent A. Post this it is to be again incubated.

·         After washing thrice, Detection Reagent B is to be added 100µL.

·         After washing for 5 times, 90µL Substrate Solution is to be added in it.

·         Finally 50µL of Stop Solution is added to make a final end to this process.

With a detection technique of Colorimetric - 450nm, results derived from Human Suppressors of Cytokine Signaling 3(SOCS3) ELISA Kit is almost accurate.