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If the user do this assay for the first time, it is recommended to do a pre-test with two strips. A suggested plate format for pre-test is shown below. This plate format is just available for cell supernatant, tissue homogenate,urine??or serum/plasm where the recommended dilution is not provided in the manual. The user may vary the location and type of wells present as necessary for each particular experiment.

 

support sample

 

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