Human Pre B cell enhancing factor (PBEF) ELISA KitHuman
serum, plasma, tissue homogenates, cell culture supernates
Principle of test
The kit is for the quantitative level of PBEF in the sample, adopt purified Human PBEF to coat microtiter plate, make solid-phase antibody, then add PBEF to wells, Combine PBEF antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of PBEF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Chromogen Solution A(1×6ml)
Chromogen Solution B(1×6ml)
Wash Solution(1×20ml×30 fold)
For Research Use Only!
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