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The kit is for the quantitative level of SOCS3 in the sample, adopt purified Human SOCS3 to coat microtiter plate, make

solid-phase antibody, then add SOCS3 to wells, Combine SOCS3 antibody with labeled HRP to form antibody-antigen

-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color

at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a

wavelength of 450 nm. The concentration of SOCS3 in the samples is then determined by comparing the O.D. of the

samples to the standard curve.

TAG:  Suppressors Of Cytokine Signaling 3 (SOCS3 )