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The kit is for the quantitative level of PP in the sample, adopt purified Human PP to coat microtiter plate, make solid-phase

antibody, then add PP to wells, Combine PP antibody with labeled HRP to form antibody-antigen -enzyme-antibody

complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP

enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a

wavelength of 450 nm. The concentration of PP in the samples is then determined by comparing the O.D. of the samples to

the standard curve.

TAG:  Human  Pepsin(PP)  ELISA Kit